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histone h2b coding sequence  (Addgene inc)


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    Structured Review

    Addgene inc histone h2b coding sequence
    Histone H2b Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histone+h2b+coding+sequence/H2B-GFP+(Plasmid+%2311680)/pm41895260-276-1-8
    Average 95 stars, based on 199 article reviews
    histone h2b coding sequence - by Bioz Stars, 2026-10
    95/100 stars

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    Sequencing:

    Article Title: Intensity Modulation of Trichromatic Split Fluorescent Proteins for Live Cell Mapping
    Article Snippet: Additional split fluorescent protein variants were generated through site-directed mutagenesis of existing templates: Cerulean 1-10 ( ) and YFP1 1-10 ( ) from sfGFP 1-10 , mNeonGreen3K 1-10 ( ) from mNeonGreen3A 1-10 , and sfCherry3C 1-10 ( ) from sfCherry2 1-10 (Addgene: #82603). .. The Histone H2B coding sequence was subcloned from Addgene (plasmid # 11680 for Histone H2B; Cambridge, MA). ..

    Article Title: Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.
    Article Snippet: The following cDNAs were synthesized with optimized codons by GeneArt (Thermo Fisher Scientific, Waltham, MA): sfGFP1-10, 3 CFP21-10, 39 mNeonGreen3A1-10, 11 sfCherry3Csp1-10, mRuby41-10, 10 GB1,18 ΔSUMOstar.19 Additional split fluorescent protein variants were generated through site-directed mutagenesis of existing templates: Cerulean1-10 10 and YFP11-10 39 from sfGFP1-10, mNeonGreen3K1-10 11 from mNeonGreen3A1-10, and sfCherry3C1-10 12 from sfCherry21-10 (Addgene: #82603). .. The Histone H2B coding sequence was subcloned from Addgene (plasmid # 11680 for Histone H2B; Cambridge, MA). .. Vector construction for stable cell line generation A base Tol2 transposon vector (pT2A-IRES-iRFP670) was constructed by subcloning iRFP670 cDNA40 with an internal ribosome entry site (IRES)41 into the pT2A vector.37 CFP21-10 cDNA was subsequently PCR-amplified and inserted into this base vector using InFusion assembly (Clontech, Mountain View, CA) to generate pT2A_CFP21-10-IRES-iRFP670.

    Plasmid Preparation:

    Article Title: Intensity Modulation of Trichromatic Split Fluorescent Proteins for Live Cell Mapping
    Article Snippet: Additional split fluorescent protein variants were generated through site-directed mutagenesis of existing templates: Cerulean 1-10 ( ) and YFP1 1-10 ( ) from sfGFP 1-10 , mNeonGreen3K 1-10 ( ) from mNeonGreen3A 1-10 , and sfCherry3C 1-10 ( ) from sfCherry2 1-10 (Addgene: #82603). .. The Histone H2B coding sequence was subcloned from Addgene (plasmid # 11680 for Histone H2B; Cambridge, MA). ..

    Article Title: Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.
    Article Snippet: The following cDNAs were synthesized with optimized codons by GeneArt (Thermo Fisher Scientific, Waltham, MA): sfGFP1-10, 3 CFP21-10, 39 mNeonGreen3A1-10, 11 sfCherry3Csp1-10, mRuby41-10, 10 GB1,18 ΔSUMOstar.19 Additional split fluorescent protein variants were generated through site-directed mutagenesis of existing templates: Cerulean1-10 10 and YFP11-10 39 from sfGFP1-10, mNeonGreen3K1-10 11 from mNeonGreen3A1-10, and sfCherry3C1-10 12 from sfCherry21-10 (Addgene: #82603). .. The Histone H2B coding sequence was subcloned from Addgene (plasmid # 11680 for Histone H2B; Cambridge, MA). .. Vector construction for stable cell line generation A base Tol2 transposon vector (pT2A-IRES-iRFP670) was constructed by subcloning iRFP670 cDNA40 with an internal ribosome entry site (IRES)41 into the pT2A vector.37 CFP21-10 cDNA was subsequently PCR-amplified and inserted into this base vector using InFusion assembly (Clontech, Mountain View, CA) to generate pT2A_CFP21-10-IRES-iRFP670.



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